In Germany, the virus can be obtained from the German Association for the Control of Viral Diseases [Deutsche Vereinigung zur Bekämpfung der Viruskrankheiten (DVV)].
Another source is the National Institute for Biological Standards and Control (NIBSC), offering the WHO reference strain Sabin Original Virus, second passage (SO 2) (NIBSC, code 01/528).
In our laboratory, the original Sabin virus obtained from Behringwerke AG is used.
In this study we used strains from all these three different sources to show that their properties are identical.
Both attenuated vaccine strains for poliovirus type 1, CHAT and Sabin, were derived from the wild Mahoney strain.
During the 1950 s, several virus variants were derived from the Mahoney strain by successive passages in various in vivo and in vitro cell substrates and selected for reduced neurovirulence in monkeys, to be used as vaccines .
The CHAT vaccine strain was produced by Hilary Koprowski at The Wistar Institute of Anatomy and Biology, Philadelphia.
The best-known vaccine strain is probably the Sabin poliovirus type 1 strain (LSc-2ab), developed by Albert Bruce Sabin at the University of Cincinnati College of Medicine and Children's Hospital Research Foundation.In a previous study, the immunity status of 10 members of the laboratory staff with OPV history against CHAT was analysed using neutralisation test to estimate the potential risk of laboratory-associated infections and transmission to the community.Seven of the samples had the antibody titres against CHAT more than twofold lower than those against poliovirus type 1 Sabin, and three of them had the titres less than 8 [Group I included sera from 41 adults.In Germany, vaccination against poliomyelitis was introduced in 1962 using the oral polio vaccine (OPV) containing Sabin strain LSc-2ab.The vaccination schedule was changed from OPV to an inactivated polio vaccine (IPV) containing wild polio virus type 1 strain Mahoney in 1998.